Abstract Details

Presented By: Davis, Tyler
Affiliated with: University of Utah, Biomedical Engineering
Authors: Davis TS, Torab K, Greger B
From: University of Utah
Title
A less invasive approach to long-term head fixation in behaving non-human primates
Abstract

Experiments in behaving non-human primates often require head fixation for accurate tracking of eye movements and stable neural recordings. A common method of head fixation uses the acrylic head cap. This procedure involves invasive surgery, which exposes the animal’s skull and increases the likelihood of infection. Additionally, a failed head cap can be traumatic to the animal, will result in a long-term interruption of the behavioral experiments, and requires invasive surgery in order to correct. We have developed an improved head restraining device that involves a simple surgery for placement. This design is similar to a human approved device used for cervical traction and stabilization. This new technique reduces the risk of infection, is much less traumatic in the event of a failure, and is easily removed for imaging. It consists of an aluminum halo, four titanium skull pins, and four reinforcing titanium plates with screws. The surgery involves four small incisions on the scalp, blunt dissection of the muscle to the skull surface, placement of the reinforcing plates, insertion of the pins for attachment of the halo, and closure of the incision sites. We currently have installed this device on two animals. These animals were able to perform trained behavioral tasks within two days of recovery. One animal has been head fixed for over two months and shows no sign of infection. We have successfully tracked the eyes of both animals to within two degrees of visual angle. A long-term investigation of this device will be performed to reaffirm its stability. Moreover, we will look into the ease of halo removal and replacement for imaging studies.