Data from neurons in vivo have shown that spike output can often sustain episodes of high variability. Theoretical studies have indicated that the high conductance state of neurons brought on by synaptic activity can contribute to an increase in the variability of spike output by decreasing the integration time scale of the neuron. In the present work, we were interested in understanding how background synaptic conductance activity uniquely alters the interspike interval (ISI) variability of layer III pyramidal cells of the medial entorhinal cortex. We compared ISI variability in pyramidal cells as a result of synaptic current- or conductance-mediated membrane fluctuations. We find that the ability of background synaptic conductance activity to increase ISI variability depends on the neuron type. In pyramidal cells lacking spike frequency adaptation, the variability increased in relation to a comparable synaptic current stimulus. In contrast, in pyramidal cells displaying spike frequency adaption, the synaptic conductance stimulus produced lower ISI variability. To understand this result we constructed a phenomenological model that reproduced the basic properties of these neurons under control and increased leak conductance. We find that leak can change the properties of the neuron by acting as a bifurcation parameter that reduces the afterdepolarization (ADP) and decreases the slope (gain) of the frequency-current relationship. A lower gain with the added leak causes a reduction in ISI variability. Hence, the ability of a high conductance state to increase ISI variability cannot be generalized and can depend on the spike ADP dynamics expressed by the neuron.